英文名稱
Dihydrofluorescein diacetate
Smiles Code
O=C(C1=C(C2C3=CC=C(C=C3OC4=CC(OC(C)=O)=CC=C42)OC(C)=O)C=CC=C1)O
Research Tools; Molecular Biology; Dyes;
Dihydrofluorescein diacetate is a chemical used for intracellular staining. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as 2',7'-dichlorodihydrofluorescein and Dihydrorhodamine 123. Dihydrofluorescein diacetate is first hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by hydrogen peroxide.